Dr. Harendra Modak
M.Sc., Ph.D.
Trained Graduate Teacher (TGT), Biology- Chemistry
……………………………………………………………………………………………………………………………………
PhD (Applied Genetics)
Topic: Expression Profiling and Mutation Analysis of PI3Kinase Pathway Genes in Acute & Chronic Myeloid Leukemia Institution: Karnataka Institute for DNA Research (KIDNAR), Karnatak University, Dharwad.
Techniques: DNA & RNA isolation, PCR, RT- qPCR, Microarray, Cytogenetics, Sequencing,
FISH PUBLICATIONS:
Harendra Modak, Tehrim Sayad, Nayana Fernandes (2016). Molecular detection of HPV 16 in saliva of patients with Oral squamous cell carcinoma and in the normal Population of southwesterly Indians. Int. J Adv. Bio. Res.; Vol. 6(1) 2016: 25-29.
Harendra Modak, Sujayendra Kulkarni, Suyamindra S. Kulkarni, Prabhanjan Gai, Umesh Hallikeri, Pramod B Gai (2014). Microarray based comparative genome -wide expression profiling of major subtypes of leukemia. Afr. J. Biotechnol; 13(10), pp. 1174-1181.
Harendra Modak, Sujayendra SK, Suyamindra SK, Pramod BG (2013). Early Diagnosis of Leukemia by Microarray Analysis for Ameliorate Suffering and Reducing Mortality of
Patients. J Pub Health Med Res; 1(1):32-36.
Suyamindra SK, Bhushan BK, Vandana T, Sujayendra SK, Gurushantappa SK, Harendra Modak, Rashmi Rasalkar, Suresh B, Prakashgoudar HK, Shivaprakash VH, Basavaraj RP, Tipperudra AS, Vijayachandra B, Muralidhar LK, Kumarasamy Thangaraj, Pramod BG (2012). Expression Profiling of brca1 Gene in Familial Breast Cancer in India. Afr. J. Biotechnol.; 11(37):9123-9126.
Suyamindra SK, Bhushan BK, Vandana T, Sujayendra SK, Gurushantappa SK, Harendra Modak, Rashmi Rasalkar, Suresh B, Prakashgoudar HK, Shivaprakash VH, Basavaraj RP, Ashalatha KV, Tipperudra AS, Vijayachandra B, Muralidhar LK, Kumarasamy Thangaraj, Pramod BG (2012). A Comparative Study of cDNA Microarray Analysis of Familial and Sporadic Breast Cancer in India. J. Med. Genet. Genomics; 4(1): 6 – 12.
Harendra Modak, Suyamindra SK, G.S.Kadakol, S.V.Hiremath, B.R.Patil, Umesh Hallikeri, Pramod BG (2011). Prevalence and Risk of Leukemia in the Multi-ethnic Population of North Karnataka. Asian Pacific J Cancer Prev; 12:1-6.
BOOK CHAPTERS:
Harendra Modak, Madhuri Biradar, Nalina M, Chandrashekara KN (2015). Chapter 7: Medical Biotechnology, Basic Concept of Biotechnology; ISBN: 978-1-312-87694-1. pp 323-346.
Harendra Modak, Chidanand Rabinal, Madhuri Biradar, Nalina M and Chandrashekara KN (2015). Chapter 8: Tools and Techniques in Biotechnology, Basic Concept of Biotechnology; ISBN: 978-1-312-87694-1. pp-347-417.
Harendra Modak and Madhuri Biradar (2015). Chapter 10: Biotechnology in Forensic Sciences, Basic Concept of Biotechnology; ISBN: 978-1-312-87694-1. pp 459-507.
Gururaj D. Kulkarni, Harendra Modak, Pramod B. Gai (2017). Need for DNA based technologies for Police, Police in the Contemporary Society. ISBN: 978-81-933650-0-7. pp 71-94.
DETAILS OF PREVIOUS EMPLOYMENTS:
WORK EXPERIENCE: 1
Institution : MedGenome Labs Pvt. Ltd., Oncology Department, Narayana Health City, Bengaluru,
Karnataka-99
Role : Associate Scientist
Responsibilities:
Analysis and interpretation of molecular genetic results (includes NGS tests and any other platforms such as Realtime PCR as and when required) for Liquid tumors (all types of Leukemia) and Solid tumors (Lung, Colorectal, GIST, Breast, Melanoma, Prostate, Renal,
Cervix etc.) for different panels.
Collaborate with expert team in the interpretation of NGS analysis and genetic diagnosis testing.
Scientific content writing for clinical project proposals and communications.
Report writing, clinical report generation and Turn-around time (TAT) maintenance.
Closely work with the genome analyst team and customer support to close projects and test orders within TAT.
Liaise with the sales and BD team to clear customer queries.
Management of Laboratory Information Management System (LIMS) status.
Work closely with management team and sales force to continuously improve our services.
Provide consultations on specific projects.
Participation in laboratory accreditation process relevant to molecular pathology/oncologythat includes analysis of results, verification and proper maintenance of records.
Literature review on various developments in oncology diagnostics and translational research and periodic presentations of the same, with focus on:
a) Coming up with new diagnostic tests in oncology related to diagnosis, prognosis and therapeutic relevance
b) Planning and validation of new molecular genetic tests in oncology
Taking responsibility in various other activities in terms of routine laboratory maintenance, following GCP and GLP guidelines, and projects as and when required.
Publish clinical and laboratory findings in peer reviewed journals, wherever required.
Any other responsibilities assigned by the reporting manager or management
WORK EXPERIENCE: 2
Institution : Karnataka Institute for DNA Research (KIDNAR), Karnatak University Campus, Dharwad, Karnataka.
Title : Centre of Excellence in Molecular Hemato Oncology, Vision Group of Science and Technology, Department of Science and Technology (VGST-DST), GoK.
Role : Senior Research fellow
Responsibilities:
1 : Visit to various hospitals and clinics for tissue and blood sample collection.
2 : Establishing collaboration and co-operation between KIDNAR and hospitals’ administration for the supplying of Leukemia Samples.
3 : Setting up wet-lab experiments, Isolation of DNA &RNA, RT-PCR, PCR amplification, Sequencing (ABI-3500), Cytogenetics.
4 : Data Analysis, Chemical procurement, Paper writing and communicating with journals.
WORK EXPERIENCE: 3
Institution : Department of Applied Genetics, Karnatak University, Dharwad, Karnataka.
Role : Teaching M.Sc students as Guest Faculty for 3 years
Responsibilities:
1 : Teaching Molecular Biology, Immunology, Biochemistry, Genetic Counseling
2 : Setting up experiments in Lab and training M.Sc. Students
3 : Guiding Final year M.Sc students in their 6 months’ projects and dissertation
WORK EXPERIENCE: 4
Institution : Department of Biotechnology, Indian Institute for Horticultural Research
(IIHR), Bangalore.
Title : DBT Project & ICAR Network Projects: Regeneration, Transformation and Development of transgenic vegetables by Bt gene stacking and gene targeting & Molecular analysis of putative transformants of vegetables.
Role : Senior Research fellow
Responsibilities:
1 : Standardization and Preparation of Tissue culture media for explants of Tomato and Brinjal.
2 : Gene transformation (Agrobacterium-mediated and Electroporation), Blotting techniques, Bio assay of putative transgenic plants.
3 : Preparation of media for maintenance of larvae of Leucinodes orbonalis,the eggplant fruit and shoot borer and the cotton bollworm or Helicoverpa armigera.
4 : Conducting field trials in green houses for putative transforms exposing them to disease pathogens.
5 : Collection and analysis of Data and overall lab supervision.
PROJECT BRIEFS (DURING M.Sc.):
Research experience in Biopesticide lab, Division of Biotechnology, Indian Institute of Horticultural Research, Bangalore, from July 2006 –April 2007.
Project entitled “Modulation of protein kinase C isoform (ε) by using natural phenolic compounds on MCF-7 cell lines” in Environmental Carcinogenesis and Toxicology lab from Chittaranjan National Cancer Institute, Kolkata, Jan 2006.
Project entitled “Isolation & characterization of α-amylase from Bacillus subtilis” from Sangene Biotech Pvt. Ltd., Bangalore, July 2005.
Advance diploma course in Molecular Biology & Genetic Engineering from Sangene Biotech Pvt. Ltd, Bangalore, Jan 2005.
HANDS-ON EXPERIENCE:
PCR, RT- qPCR, Microarray, Cytogenetics, Sequencing, FISH
Animal & Plant Tissue culture
Gene transformation (Agrobacterium-mediated and Electroporation)
Isolation and sizing of DNA, RNA and proteins from human and plant
Isolation & identification of microorganisms from soil
Biochemical tests and Mass production of B. subtilis by solid state fermentation
Extraction and estimation of amylase by DNS method, Ammonium Sulphate precipitation and dialysis of enzyme
Purification of enzyme by DEAE cellulose from column chromatography
Effect of substrate, temperature & pH on activity of α amylase
SDS-page & immobilization of enzyme
Isolation and estimation of protein by spectrophotometer, haemocytometer and Bronsted-Lowry method
Western blot analysis.
Protein purification by ion-exchange, column and affinity chromatography.
Blotting techniques, Bio assay of putative transgenic plants
ORAL PRESENTATIONS:
Harendra Modak, et al. Gene expression profiling of four types of Leukemia based upon microarray analysis and sorting out differentially expressed genes (OP-41). National Conference on Environmental Sciences and Health Hazards; 19-21 Nov 2012.
Vageeshbabu S. Hanur, Harendra Modak, PM Shamseer, A Purushothama, R Asokan. Development of cry2A Bt transgenic eggplant for resistance to the shoot and fruit borer, Leucinoides orbonalis Guenee (1.1-O6b). International Conference on Horticulture (ICH 2009) organized by PNASF, VEGINET and UAS; 9-12 Nov 2009.
POSTER PRESENTATIONS:
Harendra Modak, et al. Early diagnosis of Leukemia (Blood Cancer) by microarray analysis for reducing mortality and ameliorate suffering (PP-8). 5th Annual KSTA Conference on Science and Technology for Societal Transformation; 19-20 Dec 2012.
Harendra Modak, et al. Comparison of frequency and risk of HPV type 16 & 18 in saliva of normal population and patients suffering from oral squamous cell carcinoma in Karnataka (PP- 14). National Conference on Environmental Sciences and Health Hazards;
19-21 Nov 2012.
Harendra Modak, et al. Microarray based gene expression profiling of major sub types of Leukemia. 3rd International Conference of Stem Cells and Cancer; 27-30 Oct 2012.
Harendra Modak, et al. Mesenchymal Stem Cells (MSCs) and its role in the treatment of Diabetes. 3rd International Conference of Stem Cells and Cancer; 27-30 Oct 2012.
Harendra Modak, et al. Earthworm in Cancer therapeutics – A current status. 3rd International Conference of Stem Cells and Cancer; 27-30 Oct 2012.
Harendra Modak, et al. “Towards development of Bt transgenic tomato: Media standardization and regeneration” in “3rd National Symposium on Plant Protection in Horticultural Crops: Emerging Trends and Challenges” held in IIHR, Bangalore, India. Pp
76.
Participated in the IIIrd National Symposium on “Plant Protection in Horticulture: Emerging Trends and Challenges” held during 7-9 March 2007 in IIHR, Bangalore. SEMINARS ATTENDED:
Attended two days of 7th All India Students’ Conference on Science and Spiritual Quest
(AISSQ-2012) held on 1-2 Sept 2012 at J N Tata Auditorium, IISc Bangalore, India.
Attended a two-day seminar on “Education towards Man- Making” held on 27-28 Feb 2011 at Swami Vivekananda Centre and Department of Education, Karnatak University, Dharwad, Karnataka.
Participated in the UGC sponsored seminar on “Promotion and Protection of Human Rights” held on 28th August 2010 at P.C. Jabin Science College, Hubli, Karnataka.
Participated in a group meeting on “Application of Bioinformatics for Pest and Disease Management” held on 7th November 2008 in IIHR, Bangalore.